Fluorescence Spectroscopy: Basics and Advanced Approaches -- Basics of Fluorescence Spectroscopy in Biosciences -- Pulse and Phase Fluorometries: An Objective Comparison -- Non-Exponential Fluorescence of Electronically Coupled Donors Contains Distance Information -- Fluorescence Nanotomography: Recent Progress, Constraints and Opportunities -- Solvent Relaxation as a Tool for Probing Micro-Polarity and -Fluidity -- Total Internal Reflection Fluorescence Microscopy: Applications in Biophysics -- Single Molecule Spectroscopy: Basics and Applications -- Application of Fluorescence Spectroscopy to Biological Membranes -- Raft Microdomains in Model Membranes as Revealed by Fluorescence Quenching -- The Lateral Structure of Lipid Membranes as Seen by Fluorescence Microscopy -- Application of Fluorescence Spectroscopy to Protein Studies -- Protein Dynamics and Protein Folding Dynamics Revealed by Time-Resolved Fluorescence -- Time-Resolved Fluorescence and Two-Photon FCS Investigation of the Interaction of HIV-1 Nucleocapsid Protein with Hairpin Loop Oligonucleotides -- Application of Fluorescence Spectroscopy to DNA and Drug Delivery -- Fluorescence Techniques in Non-Viral Gene Therapy -- Fluorescence Applications in Targeted Drug Delivery -- Fluorescence Spectroscopy in cells: FCS and Quantum Dots -- Fluorescence Correlation Spectroscopy in Cell Biology -- Fluorescent Quantum Dots: Properties and Applications -- Heat Stress of Cancer Cells: Fluorescence Imaging of Structural Changes with Quantum Dots 605 and Alexa 488.